Like the original LocoRoco game, the player controls are the same, as they once again play as the planet. The pink blob gelatin daughter named Lucy.
Noticeable new characters in the game include Bonmucho's mother, Majolinè, the BuiBui (who were MuiMui, but got kissed by Majolinè, turning them evil and have a red color to distinguish themselves from the MuiMui), Viole, a new purple LocoRoco, and an old lady named Galanmar. LocoRoco 2 has many new features added from its previous game, including the ability to swim underwater, squeeze through crevices, and gain many new abilities. The LocoRoco then set off on an, even more, epic journey to restore the life force into living things and to defeat the Moja Corps. Back there, the LocoRoco finds the new MuiMui house, but right after, the meteor comes crashing down onto a Nyokki, and the Mojas start attacking again (sucking the life force out of living things, and as usual, eating LocoRoco). Armed with this fearsome song, the Moja boarded their meteorite and set off once more on a mission to conquer the LocoRoco planet. However, Bon Mucho, the Moja Boss, is not willing to accept defeat, so he devises a terrible song that can suck the life force out of living things, as a new attack on the LocoRoco. Having successfully defeated the Moja Corps, the LocoRoco settle back in their peaceful life.